Professor

Vice Director, National Biomedical Imaging Center

National Outstanding Young Scholar

Biography

Liangyi Chen is Boya Professor of Peking University. He obtained his undergraduate degrees Biomedical engineering in Xi’an JiaoTong University, then majored in Biomedical engineering in pursuing PhD degree in Huazhong University of Science and Technology. His lab focused on two interweaved aspects: the development of new imaging and quantitative image analysis algorithms, and the application of these technology to study how glucose-stimulated insulin secretion is regulated in the health and disease at multiple levels (single cells, islets and in vivo) in the health and disease animal models. The techniques developed included ultrasensitive Hessian structured illumination microscopy (Hessian SIM) for live cell super-resolution imaging, the Sparse deconvolution algorithm for extending spatial resolution of fluorescence microscopes limited by the optics, Super-resolution fluorescence-assisted diffraction computational tomography (SR-FACT) for revealing the three-dimensional landscape of the cellular organelle interactome, two-photon three-axis digital scanned lightsheet microscopy (2P3A-DSLM) for tissue and small organism imaging, and fast High-resolution Miniature Two-photon Microscopy (FHIRM-TPM) for Brain Imaging in Freely-behaving Mice. He is recipient of the National Distinguish Scholar Fund project from National Natural Science Foundation of China, and also a guest professor at Université PSL and École Normale Supérieure.

Courses

n Advanced Biophysics (2 credits)

n Biofluorescence Imaging (2 credits)

n Islet cell biology (2 credits)

Education

n BE 1995 Biomedical engineering in Xi’an JiaoTong University

n Ph. D.  2001 Biomedical engineering in Huazhong University of Science and Technology (Advisor: Prof. Hua-Guang Kang)

Experience

n 2001. 4 – 2003.6  Postdoc fellow, University of Washington, Seattle (Advisor: Bertil Hille)

n 2003. 6 – 2010.3  Associate Professor, Institute of Biophysics, Chinese Academy of Sciences, Beijing  

n 2010. 3 – 2017.7  Principal Investigator, IMM, Peking University, Beijing

n 2017. 7 – 2019.6  Principal Investigator with Tenure, Institute of Molecular Medicine, Peking University

n 2020. 1 – now Boya Distinguished Professor, Peking University

Projects:

n 2021.1-2022.12 Principal Investigator, the Major Research Plan from National Natural Science Foundation of China (No. 92054301)

Title: Developing dual-mode super-resolution holistic imaging technique to map out the organelle interactome during cell autophagy and migrasome fusion (RMB 9,000,000)

n 2020. 1 – 2023.12 Principal Investigator, National Distinguish Scholar Fund from National Natural Science Foundation of China (No. 81925022)

      Title: Biomedical Photonics (RMB 4,000,000)

Representative publications:

1. Zhao W, Zhao S, Li L, Huang X, Xing S, Zhang Y, Qiu G, Han Z, Shang Y, Sun D, Shan C, Wu R, Gu L, Zhang S, Chen R, Xiao J, Mo Y, Wang J, Ji W, Chen X, Ding B, Liu Y, Mao H, Song B, Tan J, Liu J, Li H*, Chen L*. Sparse deconvolution improves the resolution of live-cell super-resolution fluorescence microscopy, Nat Biotech., 2021. Nov 15. doi: 10.1038/s41587-021-01092-2. Highlighted as "Technical advance" in Faculty Opinions and selected as "2021 Top 10 socially influential events in the field of optics in China (Light10) "

2. Zong W, Wu R*, Chen S, Wu J, Wang H, Zhao Z, Chen G, Tu R, Wu D, Hu Y, Xu Y, Wang Y, Duan Z, Wu H, Zhang Y, Zhang Y, Wang A*, Chen L*, Cheng H. Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging, Nat Methods. 2021 Jan;18(1):46-49.

3. Zheng X, Duan R, Li L, Xing S, Ji H, Yan H, Gao K, Wang J, Wang J*, Chen L*. Live-cell superresolution pathology reveals different molecular mechanisms of Pelizaeus-Merzbacher disease, Science Bulletin, 30 December 2020; 65(24): 2061-2064.

4. Zhang Y, Wang J, Xing S, Li L, Zhao S, Zhu W, Liang K, Liu L, Chen L*, Mitochondria determine the sequential propagation of the calcium macrodomains revealed by the super-resolution calcium lantern imaging, Sci China Life Sci. 2020 Oct;63(10):1543-1551.

5. Dong D, Huang X, Li L, Mao H,Mo Y, Zhang G,Zhang Z, Shen J, Liu W, Wu Z,Liu G, Liu Y, Yang H, Gong Q, Shi K*, Chen L*, Super-resolution fluorescence-assisted diffraction computational tomography reveals the three-dimensional landscape of the cellular organelle interactome, Light Sci Appl. 2020 Jan 28;9:11. doi: 10.1038/s41377-020-0249-4. eCollection 2020.

6. Zhao J, Zong W, Zhao Y, Gou D, Liang S, Shen J, Wu Y, Zheng X, Wu R, Wang X, Niu F, Wang A, Zhang Y, Xiong JW, Chen L*, Liu Y*. In Vivo imaging of β-cell function reveals glucose-mediated heterogeneity of β-cell functional development. Elife. 2019 Jan 29;8. pii: e41540. doi: 10.7554/eLife.41540. Highlighted in the Nature Reviews Endocrinology (2019). https://doi.org/10.1038/s41574-019-0181-y;

7. Huang X, Fan J, Li L, Liu H, Wu R, Wu Y, Wei L, Mao H, Lal A, Xi P, Tang L, Zhang Y, Liu Y, Tan S*, Chen L*. Fast, long-term super-resolution imaging with Hessian structured illumination microscopy, Nat Biotech., 2018 Jun;36(5):451-459. Highlighted in the Nature Methods (2018) https://doi.org/10.1038/s41592-018-0023-1. Selected as “China's Top Ten Advances in Optics in 2018”; Video: https://www.youtube.com/watch?v=U9Om7DPxzd0&feature=youtu.be

8. Zong W, Wu R, Li M, Hu Y, Li Y, Li J, Rong H, Wu H, Xu Y, Lu Y, Jia H, Fan M, Zhou Z, Zhang Y*, Wang A*, Chen L*, Cheng H. Fast High-resolution Miniature Two-photon Microscopy for Brain Imaging in Freely-behaving Mice. Nat Methods. 2017 Jul;14(7):713-719. An exclusive commentary in Light: Science & Applications (2017) 6, e17104. Selected as “China's Top Ten Advances in Science in 2017”, “China's Top Ten Medical Science and Technology News in 2017” and “China's Top Ten Advances in Life Science in 2017”. In 2018, the method was selected by Nature Methods as "Method of the Year 2018"; Video: https://www.youtube.com/watch?v=AED0SJVAlp0

9. Yuan T, Liu L, Zhang Y, Wei L, Zhao S, Zheng X, Huang X, Boulanger J, Gueudry C, Lu J, Xie L, Du W, Zong W, Yang L, Salamero J, Liu Y*, Chen L*. Diacylglycerol Guides the Hopping of Clathrin-Coated Pits along Microtubules for Exo-Endocytosis Coupling. Dev Cell. 2015 Oct 12;35(1):120-30. Previewed in the same issue of Dev Cell, highlighted in the F1000Prime as NEW FINDING and INTERESTING HYPOTHESIS and also highlighted on the American Society of Cell Biology (ASCB) website.

10. Zong W, Zhao J, Chen X, Lin Y, Ren H, Zhang Y, Fan M, Zhou Z, Cheng H, Sun Y*, Chen L*. Large-field high-resolution two-photon digital scanned light-sheet microscopy. Cell Res. 2015 Feb;25(2):254-7