Associate Professor (with Tenure)

Department of Biomedical Engineering

Biography

Dr. Antony K. Chen is an associated professor in College of Future Technology, Peking University, China. His major research interests lie in the fields of nucleic acid nanotechnology and single molecule live-cell imaging. He has published over 40 articles in journals such as Proc Natl Acad Sci U S A, Nucleic Acids Research and ACS Nano, and delivered talks on international conferences such as BMES, WMIC and CSHL.

Courses

n Cellular and Molecular Imaging (UG, 3 credits)

n Introduction to Biomedical Engineering (G, 3 credits)

Education

n B.S. 2000 Bioengineering, University of California, San Diego

n M.S.  2002 Bioengineering, University of California, San Diego (Thesis Advisor: Dr. John A. Frangos)

n Ph.D. 2008 Bioengineering, University of Pennsylvania (Thesis Advisor: Dr. Andrew Tsourkas)

Professional Experiences

n 2009.1 – 2010.1  Postdoc, Department of Bioengineering, University of Pennsylvania (Mentor: Dr. Andrew Tsourkas)

n 2010.3 – 2012.3  Postdoc, National Institute of Standards of Technology, USA (Mentor: Dr. Anne Plant)

n 2010.3 – 2013.3   Postdoc, National Institutes of Health, USA (Mentor: Dr. Jennifer Lippincott-Schwartz)

n 2013. 4 – 2019.7  Assistant Professor, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China

n 2019. 8 – 2021.3 Associate Professor, Department of Biomedical Engineering, College of Engineering, Peking University, Beijing, China

n 2021. 4 – Present Associate Professor, Department of Biomedical Engineering, College of Future Technology, Peking University, Beijing, China

Projects:

n 2021.12 – 2026.6 Principal Investigator, National Key R&D Program of China (No. 2021YFA1100201)

Title: Developing analytical approaches to decipher dynamic organizations and localizations of membraneless organelles key to stem cell fate determination (RMB 2,764,300)

n 2022. 1 – 2025.12 Principal Investigator, National Natural Science Foundation of China (No. 22177001)

Title: Development and applications of a live-cell imaging platform for intracellular mitochondrial RNA analysis (RMB 600,000)

Representative publications:

1. Qu, N., Ying, Y., Qin, S., and Chen, A.K.* Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockade. Nucleic Acids Res. 2022; 50, e44.

2. Mao, S., Ying, Y., Zhao, M., Yang, Y., and Chen, A.K.* A Background Assessable and Correctable Bimolecular Fluorescence Complementation System for Nanoscopic Single-Molecule Imaging of Intracellular Protein-Protein Interactions. ACS Nano. 2021；15, 14338-14346.

3. Mao, S., Ying, Y., Wu, R., and Chen, A.K.* Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging. iScience. 2020; 23, 101801.

4. Mao, S., Ying, Y., Wu, X., Krueger, C.J. and Chen, A.K.* CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Nucleic Acids Res. 2019; 47, e131.

5. Ding, T., Chen, A.K.* and Lu, Z.* The applications of nanopores in studies of proteins. Sci Bull. 2019; 64, 1456-1467. (Review).

6. Wu, X., Mao, S., Yachen, Ying., Krueger, C.J. and Chen, A.K.* Progress and Challenges for Live-Cell Imaging of Single Genomic Loci Using CRISPR-Based Platforms. Genomics, proteomics & bioinformatics. 2019; 17(2): 119-128.

7. Yang, Y., Qu, N., Tan, J., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Roles of Gag-RNA interactions in HIV-1 virus assembly deciphered by single-molecule localization microscopy. Proc Natl Acad Sci U S A. 2018; 115, 6721-6726.

8. Wu, X., Mao, S., Yang, Y., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. 2018; Nucleic Acids Res, 46, e80.

9. Qu, N., Ma, Z., Zhang, M., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation. Protein Cell. 2018; 9, 640-651.

10. Zhao, D., Yang, Y., Qu, N., Chen, M., Ma, Z. Krueger, C.J., Behlke, M.A. and Chen, A.K.* Single-Molecule Detection and Tracking of RNA Transcripts in Living Cells Using Phosphorothioate-Optimized 2’-O-Methyl RNA Molecular Beacons. Biomaterials. 2016; 100: 172-183.