Abstract

Life unfolds in three dimensions (3D),and fully understanding tissue function and disease requires methods that preserve spatial integrity.3D imaging now enables visualization of RNA transcripts throughout intact biological samples, revealing the complete spatial organization of complex organs.

In this presentation, I will describe the development of TRISCO,a technology for whole-brain 3D spatial transcriptomics that enables imaging of RNA transcripts throughout intact tissues at single-cell resolution. By combining tissue clearing, RNA labeling, light-sheet microscopy, and computational image analysis, TRISCO generates comprehensive molecular maps while preserving the native 3D organization of the brain.

I will present the development of TRISCO, which generated the first whole-brain 3D maps of multiple RNA transcripts in intact adult mouse brains, revealing region-specific gene expression patterns, long-ra cellular architecture, and molecular diversity across the nervous system. I will also discuss how TRISCO bridges the gap between single-cel genomics and systems neuroscience, providing a scalable framework for studying brain development, neural circuit organization,and neurological disease.