摘要：

Advanced whole-brain mapping is critical for addressing complex neuroscience questions, yet current imaging methods are limited by throughput, resolution, and signal-to-noise ratio.

In this presentation, we introduced Confocal Airy Beam Oblique Light-Sheet Tomography (CAB-OLST), a technique enabling high-throughput, high-resolution, and high-SNR imaging. CAB-OLST achieves precise brain-wide cell distribution mapping at 0.37 μm × 0.37 μm × 1.77 μm resolution in just 10 hours, and detailed imaging of long-distance axonal projections throughout the entire mouse brain at 0.26 μm × 0.26 μm × 1.06 μm resolution over 58 hours. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.

个人简介：

齐晓莉博士，北京生命科学研究所/清华大学生物医学交叉研究院生物仪器中心主任，入选北京市海外人才青年项目。本科和博士毕业于华中科技大学，先后于华盛顿大学、约翰霍普金斯大学及冷泉港实验室开展研究工作。主要从事生物显微光学成像技术的研究与应用，长期致力于开发新型高分辨率成像系统及其在神经科学中的应用。在Nature、Nature Methods、Cell等国际期刊发表论文16篇（第一/通讯作者4篇，其中包括Nature Methods 1篇）。曾作为美国NIH BRAIN Initiative核心技术成员，参与大型国际合作项目的关键成像技术开发。