Abstract：Fluorescence microscopy is a key tool in the life sciences. While standard super-resolution techniques can reach resolutions of 15–20 nm, interactions between biomolecules happen at much smaller scales. State-of-the-art methods like MINFLUX can achieve resolutions down to 5 nm under specific conditions, but this does not easily translate to studies in cells. We introduce a technique called Resolution Enhancement by Sequential Imaging (RESI), which improves fluorescence microscopy resolution down to the Ångström scale using standard equipment and reagents. We resolve DNA base-pairs at Ångström resolution and use it to map the molecular arrangement of the immunotherapy target CD20 in cells before and after drug treatment. Furthermore, we demonstrate Ångström-resolution imaging of glycans on the cell surface. RESI bridges the gap between super-resolution microscopy and structural biology, offering new insights into complex biological systems.

Biography: Dr. Luciano A. Masullo holds a Ph.D. from the University of Buenos Aires in Argentina (2021) and is currently a Skłodowska-Curie Postdoctoral Fellow at the Max Planck Institute of Biochemistry in Munich, Germany. He has pioneered the development of new super-resolution microscopy methods with first-authored publications in journals including Nature, Nature Communications, Nano Letters, and Light: Science and Applications.