iCANX Talks Vol 70
Sep 24th, 2021
Super-resolution: an adventure on a new dimension
Peng Xi
Department of Biomedical Engineering, College of Future Technology, Peking University, China
E-mail : xipeng@pku.edu.cn
Website : https://www.xipenglab.com
ABSTRACT
Super-resolution microscopy can attain unprecedented spatial resolution for the study of biological interactions. Here I will present two developments on the polarization-based structured illumination microscopy: (1) We developed polarized structured illumination microscopy (pSIM), which achieves super-resolution imaging of dipoles by interpreting the dipoles in spatio-angular hyperspace. We demonstrate the application of pSIM on a series of biological filamentous systems, such as cytoskeleton networks and λ-DNA, and report the dynamics of short actin sliding across a myosin-coated surface. Moreover, pSIM reveals the side-by-side organization of the actin ring structures in the membrane-associated periodic skeleton of hippocampal neurons. (2) We further report Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one single dye that universally stains lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, even within the same organelle.
BIOGRAPHY
Dr. Peng Xi is a professor in College of Future Technology, Peking University, China. His research interest is focused on research and development of optical super-resolution microscopy techniques. He has published over 70 scientific papers in peer-reviewed journals including Nature, Nature Methods, Light, etc., and holds 19 issued invention patents, including 4 US patents. He is elected as a senior member of OSA since 2015. In 2020, he was awarded the NSFC National Outstanding Young Scholar. He is currently on the editorial board of 5 SCI-indexed journals such as Light: Science and Applications and Advanced Photonics. He has been invited to give over 50 invited talks in international conferences hosted by OSA and SPIE. He is also the Managing editor for Light: Science and Applications Beijing Office, in responsible for the News and Views section of the journal.
