研究员

个人简介

陈匡时多年来致力于活细胞RNA、DNA、蛋白质原位可视化技术的开发及生物学应用。2013年4月加入北京大学后以通讯作者身份建立了可实时监测单一RNA与单基因位点动态行为的单分子RNA/DNA活细胞原位可视化技术（Live-FISH）平台，发现了microRNA能够与HIV-1和MLV病毒的结构蛋白Gag互作并干扰影响病毒颗粒的组装，并通过超分辨率荧光显微镜解析了HIV-1病毒蛋白Gag在病毒组装中与病毒RNA以及宿主细胞蛋白质的互作模式。受邀在 Genomics Proteomics & Bioinformatics, Science Bulletin，iScience等期刊发表RNA、DNA或蛋白检测的综述。

教育背景

2004-2008， 博士：宾夕法尼亚大学 (University of Pennsylvania)   专业：生物医学工程 （导师： Andrew Tsourkas） 

2000-2002， 硕士：加州大学-圣地亚哥校区  (UC San Diego)   专业：生物医学工程

1996-2000， 学士：加州大学-圣地亚哥校区 (UC San Diego)   专业：生物医学工程 

主要工作经历

2021.1-现在  北京大学未来技术学院生物医学工程系研究员、长聘副教授

2019.8-2020.12  北京大学工学院生物医学工程系研究员、长聘副教授

2013.4-2019.7 北京大学工学院生物医学工程系特聘研究员、助理教授

2010.3-2013.3  美国国家卫生研究院 (NIH)，Jennifer Lippincott-Schwartz 实验室，博士后

2010.3-2012.3   美国国家标准与技术研究院 (NIST)，Anne L. Plant 实验室，博士后

2009.1-2010.1    美国宾夕法尼亚大学，Andrew Tsourkas 实验室，博士后

代表性论文

1. Qu, N., Ying, Y., Qin, S., and Chen, A.K.* Rational design of self-assembled RNA nanostructures for HIV-1 virus assembly blockade. Nucleic Acids Res. 2021; gkab1282

2. Mao, S., Ying, Y., Zhao, M., Yang, Y., and Chen, A.K.* A Background Assessable and Correctable Bimolecular Fluorescence Complementation System for Nanoscopic Single-Molecule Imaging of Intracellular Protein-Protein Interactions. ACS Nano. 2021；15, 14338-14346.

3. Mao, S., Ying, Y., Wu, R., and Chen, A.K.* Recent Advances in the Molecular Beacon Technology for Live-Cell Single-Molecule Imaging. iScience. 2020; 23, 101801.

4. Mao, S., Ying, Y., Wu, X., Krueger, C.J. and Chen, A.K.* CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Nucleic Acids Res. 2019; 47, e131.

 5. Ding, T., Chen, A.K.* and Lu, Z.* The applications of nanopores in studies of proteins. Sci Bull. 2019; 64, 1456-1467. (Review).

6. Wu, X., Mao, S., Yachen, Ying., Krueger, C.J. and Chen, A.K.* Progress and Challenges for Live-Cell Imaging of Single Genomic Loci Using CRISPR-Based Platforms. Genomics, proteomics & bioinformatics. 2019; 17(2): 119-128.

7. Yang, Y., Qu, N., Tan, J., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Roles of Gag-RNA interactions in HIV-1 virus assembly deciphered by single-molecule localization microscopy. Proc Natl Acad Sci U S A. 2018; 115, 6721-6726.

8. Wu, X., Mao, S., Yang, Y., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. 2018; Nucleic Acids Res, 46, e80.

9. Qu, N., Ma, Z., Zhang, M., Rushdi, M.N., Krueger, C.J. and Chen, A.K.* Inhibition of retroviral Gag assembly by non-silencing miRNAs promotes autophagic viral degradation. Protein Cell. 2018; 9, 640-651.

10. Zhao, D., Yang, Y., Qu, N., Chen, M., Ma, Z. Krueger, C.J., Behlke, M.A. and Chen, A.K.* Single-Molecule Detection and Tracking of RNA Transcripts in Living Cells Using Phosphorothioate-Optimized 2’-O-Methyl RNA Molecular Beacons. Biomaterials. 2016; 100: 172-183.